Not just mammals, as far as I know it only works in E. coli bacteria and not in any eukaryotes.
Source:
Metacelsus
Karma: 1,212
How (and why) to get tested for CMV
Interestingly there was just a similar article in the news section of Science, about glacier geoengineering.
>I’m using Quaise Energy as an example of a much larger overall trend—of the inability of investors to effectively evaluate technologies. The ability of investors to recognize good technical evaluations is the key thing that’s lacking in the economy today; there are plenty of good ideas and there’s plenty of investment capital.
Yeah, just look at Varda “manufacture drugs in space,” and Colossal “bring back the wooly mammoth.” It just doesn’t make sense for these to be profitable businesses.
Reposting a comment from the Substack:
>Recently, he solved this problem!
I’m flattered, but I actually haven’t gotten all the way to haploid cells yet. As I wrote in my preprint and associated blog post, right now I can get the cells to initiate meiosis and progress about 3⁄4 of the way through it (specifically, to the pachytene stage). I’m still working on getting all the way to haploid cells and I have a few potentially promising approaches for this.
Very cool. I wonder which University of Missouri lab the lentiviral plasmid leaked from...
I went a few times but eventually got grossed out by all the mold. (At least they don’t sell live pangolins there.)
At the time, one of the biggest problems in physics was the “Blackbody spectrum”, which describes the spectrum of electromagnetic wavelengths emitted by a Blackbody. The problem with it was that the emitted spectrum was not explainable by known physics. Einstein achieved a breakthrough by considering light not just as a wave, but also as light quanta. Although this idea sufficiently explained the Blackbody spectrum, physicists (at least almost) unanimously rejected it. The fight between the “light is corpuscles” and “light is a wave” faction had been decided a century ago, with a clear victory for the “wave” faction.
I thought blackbody radiation was Planck, not Einstein.
Interestingly, many cancer “neoantigens” (for example, MAGEB1) are also expressed in meiotic cells in the testis. This is because they’re usually epigenetically suppressed in healthy tissues, but cancer cells have messed up epigenomes. See: https://en.wikipedia.org/wiki/Cancer/testis_antigens
Also, I would disagree that synthesizing long polypeptides is easier than synthesizing long mRNAs. With polypeptides you have 20 amino acids to work with, and some require special treatment (protecting groups on sidechains). With mRNAs the chemistry is much simpler.
On Minicircle
Testosterone will land you in more legal trouble than modafinil.
Compounding pharmacies are gray-market. (Buying on “evolutionpeptides.com″ would be black-market.)
No mention of modafinil? It’s quite useful for maintaining productivity on low amounts of sleep.
Semaglutide is the real-deal weight loss drug we have been praying for. It works well for 70%+ of people. Losing and keeping weight off is so difficult that prior to ozempic, it was reasonable advice to preach acceptance or extremely restricted diets. Prior to Semaglutide, I used to assume that most of my friends who wanted to lose weight would fail. Now I assume they will trivially succeed if they get on the drugs. Here is how to get on Semaglutide:
I purchased sema, for myself and others, on this site: https://evolutionpeptides.com/products/semaglutide-10mg?variant=42834747326660. It has been a reliable supplier for me. Reliability can always change, but for now, it’s where I would go.
Start with a dose of 0.25mg. Increase your dose approximately every four weeks. Stay on lower dosages as long as possible. Tolerance can increase rapidly. For example, the official guidelines say to double your dose after each of the first two months of treatment. I would try to increase dosages more slowly.
Gray market semaglutide is sold as a powder. You need to mix it into a solution to inject. Search for reconstitution solution.
You also need needles. Any insulin needle will work fine but some hurt less than others. Here are the ones I use.
To make the solution, I draw 100 units of reconstitution (a ‘full’ vial) solution into the needle. I then squirt the solution into the sema vial and repeat this process again. This means 5mg of semaglutide per 200 units of solution. So, to do a 0.25 starting dose, I would inject 10 units of mixed sema solution into my deltoid. It doesn’t really matter if you inject into fat or muscle.
Semaglutide feels weird in many ways and makes many people nauseous. Fake Dr. Sapphire’s medical advice is to use gray market odansetron to manage nausea. But dramamine is OTC in the USA and most other countries and also works well. Don’t expect insanely rapid weight loss. It’s normal to lose 1-2 pounds a week, which is honestly quite quick!
Injecting anything from the “gray-market” is quite risky, since it may not be sterile. People have died from such things (for example: https://en.wikipedia.org/wiki/New_England_Compounding_Center_meningitis_outbreak)
For biology, JoVE (“Journal of Visual Experiments”) is a very good source of videos like this. https://www.jove.com/ Unfortunately it’s paywalled.
I agree, I think the most likely version of the lab leak scenario does not involve an engineered virus. Personally I would say 60% chance zoonotic, 40% chance lab leak.
A commenter on my Substack got much better results using Claude 3 Opus.
Testing ChatGPT for cell type recognition
Spearman (rank) correlation is often a good alternative for nonlinear relationships.
200 mg/day is a pretty high dose (at least for me)