I find it great that you took up the task to run the experiment. I’m a bit curious about whether part of you getting interested in orexin was downstream from my post Orexin and the quest for more waking hours.
When I asked ChatGPT “What happens to orexin if you store it in a saline solution?” I got as a response“If you store orexin in saline, expect it to become unreliable fairly quickly.” Using as freemany detailed a low dose and additional a poor way to store it, probably resulted in the null effect.
That’s a great post! It did more to popularize the idea than I ever could. I’ve been thinking about this for a while and my first writing on the topic was in 2021. I’m going to refrain from linking to it because I’m planning on depreciating that blog soon though.
Re storage and handling: this part was tricky, we opted to dissolve in sterile water (not saline) and froze the batch after mixing. So doses were only exposed to room temperature for ~minutes. We also used a C-terminal NH2 peptide that is less susceptible to degradation.
There’s still no guarantee that peptide is stable under these conditions and we don’t have a good way to check. This is a big reason why we thought there was a ~60% chance of a null on this trial (and the next one too perhaps). But hope springs eternal!
I think this is one of the cases where a quick discussion with an LLM can be helpful to check trial protocols. ChatGPT did find https://cdn.caymanchem.com/cdn/insert/15073.pdf which suggests dissolving the Orexin first in DMSO.
I find it great that you took up the task to run the experiment. I’m a bit curious about whether part of you getting interested in orexin was downstream from my post Orexin and the quest for more waking hours.
When I asked ChatGPT “What happens to orexin if you store it in a saline solution?” I got as a response “If you store orexin in saline, expect it to become unreliable fairly quickly.” Using as freemany detailed a low dose and additional a poor way to store it, probably resulted in the null effect.
That’s a great post! It did more to popularize the idea than I ever could. I’ve been thinking about this for a while and my first writing on the topic was in 2021. I’m going to refrain from linking to it because I’m planning on depreciating that blog soon though.
Re storage and handling: this part was tricky, we opted to dissolve in sterile water (not saline) and froze the batch after mixing. So doses were only exposed to room temperature for ~minutes. We also used a C-terminal NH2 peptide that is less susceptible to degradation.
There’s still no guarantee that peptide is stable under these conditions and we don’t have a good way to check. This is a big reason why we thought there was a ~60% chance of a null on this trial (and the next one too perhaps). But hope springs eternal!
I think this is one of the cases where a quick discussion with an LLM can be helpful to check trial protocols. ChatGPT did find https://cdn.caymanchem.com/cdn/insert/15073.pdf which suggests dissolving the Orexin first in DMSO.