Still reading and thanks for the write up. Much better than I could do myself and have been thinking it’s time to revisit and see where things stand.
But think this is an obvious type so wanted to mention it for your edit. “In other words, if your biological age is lower than your biological age, you’re doing great.” I assum you mean lower than your chronoloical age there.
So was farther along than I thought. Quick question on the reprogramming aspect. Certainly tissue complexity is a problem when the reaction rates are different and we probably really need to keep something of a balance in general state of cells. Does the interval between cycles of the YF application greatly impact results? You mention a one shot treatment not realy deliering much in the way of benefit so I’m wonder if there an interval period in a cyclic treatement that effectely becomes a bunch of one shot type treatments that are really going no where?
As for the latter question, I looked into this, and I think the 2 day ‘on’ of YF expression is literally just the max time it can be expressed without deleterious effects. I think people haven’t investigated the ‘off’ cycle time as much. I suspect people are on the ‘the more reprogramming I can do, the better’ train up until recently, so that level of optimization likely is higher handing fruit.
From here: https://www.nature.com/articles/s41467-024-46020-5 ″The currently optimized method of partial reprogramming is the maturation phase partial reprogramming, which necessitates 13 days of continuous expression of Yamanaka factors in vitro...However, this optimized in vitro method may be highly damaging in in vivo models, as a continuous expression of Yamanaka factors for more than 2 days may have lethal effects in mice8,67. In vivo studies now focus on cyclic partial reprogramming....”
Still reading and thanks for the write up. Much better than I could do myself and have been thinking it’s time to revisit and see where things stand.
But think this is an obvious type so wanted to mention it for your edit. “In other words, if your biological age is lower than your biological age, you’re doing great.” I assum you mean lower than your chronoloical age there.
So was farther along than I thought. Quick question on the reprogramming aspect. Certainly tissue complexity is a problem when the reaction rates are different and we probably really need to keep something of a balance in general state of cells. Does the interval between cycles of the YF application greatly impact results? You mention a one shot treatment not realy deliering much in the way of benefit so I’m wonder if there an interval period in a cyclic treatement that effectely becomes a bunch of one shot type treatments that are really going no where?
Great catch, dumb mistake on my part, fixed!
As for the latter question, I looked into this, and I think the 2 day ‘on’ of YF expression is literally just the max time it can be expressed without deleterious effects. I think people haven’t investigated the ‘off’ cycle time as much. I suspect people are on the ‘the more reprogramming I can do, the better’ train up until recently, so that level of optimization likely is higher handing fruit.
From here: https://www.nature.com/articles/s41467-024-46020-5
″The currently optimized method of partial reprogramming is the maturation phase partial reprogramming, which necessitates 13 days of continuous expression of Yamanaka factors in vitro...However, this optimized in vitro method may be highly damaging in in vivo models, as a continuous expression of Yamanaka factors for more than 2 days may have lethal effects in mice8,67. In vivo studies now focus on cyclic partial reprogramming....”