I’ve heard talk of an intermediate step of amplifying the relevant part of the plasmid up a couple trillion times via PCR to make purification easier, such that there’s less material from the original bacteria present per unit DNA and hitting your stringent composition targets is easier.
I’ve heard talk of an intermediate step of amplifying the relevant part of the plasmid up a couple trillion times via PCR to make purification easier, such that there’s less material from the original bacteria present per unit DNA and hitting your stringent composition targets is easier.