Awesome, thanks for the detailed response. After reading about CRISPR’s natural role in bacteria I was curious if it would have targeting limitations. It sounds like it does (needs GGG triplet), but that in practice this isn’t a big deal.
You still need to get this system into a cell—that’s an issue as always, I agree—but the reduced chance of unwanted mutation seems like a big step forward over retroviruses.
Awesome, thanks for the detailed response. After reading about CRISPR’s natural role in bacteria I was curious if it would have targeting limitations. It sounds like it does (needs GGG triplet), but that in practice this isn’t a big deal.
You still need to get this system into a cell—that’s an issue as always, I agree—but the reduced chance of unwanted mutation seems like a big step forward over retroviruses.
Thanks again for the great write up!