I know you linked to places that appear to explain it—but can you give the short summary of how your approach differs/what it even is that you are doing? Just reading the post makes it unclear—do you still pump them with cryoprotectant and then cool? Do you chemically fixate the brain? Third thing?
We do both! The aldehydes chemically fix the brain (this is the big thing that sets our approach apart) and protect it from being damaged by the cryoprotectant. At that point it’s stable at room temperature on the scale of weeks to months. Cooling it down to the long-term storage temperature extends that time to centuries.
I know you linked to places that appear to explain it—but can you give the short summary of how your approach differs/what it even is that you are doing? Just reading the post makes it unclear—do you still pump them with cryoprotectant and then cool? Do you chemically fixate the brain? Third thing?
We do both! The aldehydes chemically fix the brain (this is the big thing that sets our approach apart) and protect it from being damaged by the cryoprotectant. At that point it’s stable at room temperature on the scale of weeks to months. Cooling it down to the long-term storage temperature extends that time to centuries.