I have read that some sequencing methods (nanopore) have a high error rate (comparing multiple reads can help correct this). Did you also spot-check some other genes that you have no reason to believe contain mutations to see if they look ok? Seeing a mutation in exactly the gene you expect is only damn strong evidence if there isn’t a sequencing error in every third gene.
I have read that some sequencing methods (nanopore) have a high error rate (comparing multiple reads can help correct this). Did you also spot-check some other genes that you have no reason to believe contain mutations to see if they look ok? Seeing a mutation in exactly the gene you expect is only damn strong evidence if there isn’t a sequencing error in every third gene.