Well, as purely anecdotal evidence, the (neruoscience) lab I was working in this summer analyzed our data correctly (with regard to this). Although, to be fair, I didn’t notice it until my PI pointed it out to me...
I was trying to say more than we actually could say. I was the one analyzing the data.
We were studying the growth of neurons and patterns of gene regulation under the presence of various inhibitors of the mechanism of depolarization regulation. Say we had inhibitors A and B.
We would have done expression profiles for cultures that were, for example, this:
5 mM KCl (physiological conditions);
25 mM KCl (depolarizing conditions);
25 mM KCl + inhibitor A;
25 mM KCl + inhibitor B;
25 mM KCl + inhibitor A and B;
(Upon editing, I can’t make those appear as a line by line list for some reason? Don’t know what’s up with that.)
I wanted to make comparisons between the A+B+25 cultures and the 25 mM Culture (and the 5 mM one), but you can’t do that. You can only compare A+B to it’s controls, A and B alone.
Well, as purely anecdotal evidence, the (neruoscience) lab I was working in this summer analyzed our data correctly (with regard to this). Although, to be fair, I didn’t notice it until my PI pointed it out to me...
You didn’t notice that your data was analyzed correctly?
I was trying to say more than we actually could say. I was the one analyzing the data.
We were studying the growth of neurons and patterns of gene regulation under the presence of various inhibitors of the mechanism of depolarization regulation. Say we had inhibitors A and B.
We would have done expression profiles for cultures that were, for example, this:
5 mM KCl (physiological conditions); 25 mM KCl (depolarizing conditions); 25 mM KCl + inhibitor A; 25 mM KCl + inhibitor B; 25 mM KCl + inhibitor A and B;
(Upon editing, I can’t make those appear as a line by line list for some reason? Don’t know what’s up with that.)
I wanted to make comparisons between the A+B+25 cultures and the 25 mM Culture (and the 5 mM one), but you can’t do that. You can only compare A+B to it’s controls, A and B alone.