I’m curious about the difference between the approach you described here and the DNA vaccines that were open sourced in Project MacAffee. This was the one done as a course by Zayner and co.
My amateur take at the differences:
Different peptide/subunit target
Vaccine given as protein rather than plasmid to produce the protein
Oo, I wasn’t even aware of that, thanks for the link!
That is a DNA vaccine, so it’s more similar to the mRNA vaccines we have now in that it contains genetic data of the virus that is then built by the body itself. This one seems to contain the entire S and N proteins, not just a subunit of the S protein.
DNA vaccines are more complicated than recombinant vaccines to get right and can cause serious damage if done wrong. That and the fact that the more complex a project, the more likely I’m going to procrastinate and let it die, made me stick with the simpler recombinant approach.
At least the ELISA approach to antibody testing is one I could have borrowed, though, and in hindsight I’m a bit disappointed I didn’t think of it myself.
Does a similarly-structured resource to the ones I linked exist for recombinant vaccines? I’d be curious for learning more about what would be needed to do this kind of DIY-ish project in the future.
Not as far as I know, butThought Emporium on Youtube has a lot of tutorial videos on genetic engineering. (FWIW, Stöcker himself failed to express the protein in bacteria and iirc used CHO instead. I don’t see any intrinsic reason why E.Coli shouldn’t work, but I’d probably use HEK or CHO myself given the choice)
Thanks for sharing this post-mortem!
I’m curious about the difference between the approach you described here and the DNA vaccines that were open sourced in Project MacAffee. This was the one done as a course by Zayner and co.
My amateur take at the differences:
Different peptide/subunit target
Vaccine given as protein rather than plasmid to produce the protein
Oo, I wasn’t even aware of that, thanks for the link!
That is a DNA vaccine, so it’s more similar to the mRNA vaccines we have now in that it contains genetic data of the virus that is then built by the body itself. This one seems to contain the entire S and N proteins, not just a subunit of the S protein.
DNA vaccines are more complicated than recombinant vaccines to get right and can cause serious damage if done wrong. That and the fact that the more complex a project, the more likely I’m going to procrastinate and let it die, made me stick with the simpler recombinant approach.
At least the ELISA approach to antibody testing is one I could have borrowed, though, and in hindsight I’m a bit disappointed I didn’t think of it myself.
Does a similarly-structured resource to the ones I linked exist for recombinant vaccines? I’d be curious for learning more about what would be needed to do this kind of DIY-ish project in the future.
Not as far as I know, butThought Emporium on Youtube has a lot of tutorial videos on genetic engineering. (FWIW, Stöcker himself failed to express the protein in bacteria and iirc used CHO instead. I don’t see any intrinsic reason why E.Coli shouldn’t work, but I’d probably use HEK or CHO myself given the choice)