The DEFUSE proposal that you linked to doesn’t (so far as I can tell) say anything about where the furin cleavage site work would be done. OP here includes an image that seems to show a Word document or something with a comment about that, but it isn’t obvious to me where it’s from or how it’s known to be genuine or anything.
The “uniformly spaced recognition sites and BsmBI” thing (at least, the instance of it that I’ve found) looks rather sketchy and unconvincing to me, though I’m not really competent to evaluate it (are you?). It’s possible that what I’m looking at isn’t what you’re referring to; I’m talking about a post on Alex Washburne’s Substack where he draws attention to mention in the DEFUSE proposal of “reverse genetic systems” and “infectious clone technology” (though so far as I can see neither of these is actually mentioned in the proposal itself), claims (I do not know with what evidence) that using these methods would produce unusually regularly spaced instances of certain genome subsequences that are targeted by BsmBI or a similar enzyme, and claims that the SARS-CoV-2 shows such unusually regularly spaced instances.
But (1) unless there’s something I’m missing this “specifically links WIV to” those methods only in a very weak sense (e.g., the DEFUSE proposal doesn’t in fact say that they are going to use those methods, or that it would be done at the WIV), (2) Washburne doesn’t provide any support for his claim that researchers using this technique would in fact make the relevant segments unusually uniform in length, and (3) nor does he seem to give any details of the analysis that supposeedly shows that SARS-CoV-2 has such unusually uniform segments. He makes some claims about earlier drafts of the DEFUSE proposal supposedly obtained by FOIA requests, which if correct go some way to filling these gaps a bit, but if he actually shows those or gives evidence that they’re real then I haven’t seen it.
(Note: I find the style of Washburne’s writing very offputting; it pattern-matches to e.g. creationists crowing about their nonsensical statistical arguments. Lots of expressions of triumph, pointlessly flowery writing, that sort of thing. Of course that isn’t strong evidence that Washburne is wrong in the same sort of way as the creationists are, but I find it does strongly incline me to skepticism. It’s odd that, when arguing that something previously dismissed as a conspiracy theory is probably true, it doesn’t occur to him to try not writing like a conspiracy theorist.)
Daszak’s comments say that much of the work could be done at WIV which could include the FCS. The comment is genuine AFAIK but you’ll have to chase it up yourself.
The work on BsmBI is somewhat compelling because the people who suspected it published before DEFUSE was unearthed, and then DEFUSE was found to contain an order for BsmBI. So, they sort of predicted this. And there are reasons to make the virus out of relatively uniformly long segments—it’s convenient, the tools and techniques have maximum lengths they can handle, and you want to minimize the total amount of work. So you use roughly equal length segments. However this is a fairly complicated series of claims and I don’t see it as being the big win for lab-leak—the big win is DEFUSE itself.
But zoom out a bit: we can just notice that all of this stuff only collects around Wuhan, WIV and associated facilities like State Key Laboratory of Virology at Wuhan University.
There’s no equivalent of this in all the other cities in China and I am pretty sure that if you look through the top 20 cities by population you won’t find something like DEFUSE. I tried this a bit with chatGPT, Google Scholar, Google Search etc. There simply are not 50 other Daszaks out there doing GoF bat coronavirus research in every other city in China. Wuhan is THE PLACE where this happens.
The DEFUSE proposal that you linked to doesn’t (so far as I can tell) say anything about where the furin cleavage site work would be done. OP here includes an image that seems to show a Word document or something with a comment about that, but it isn’t obvious to me where it’s from or how it’s known to be genuine or anything.
The “uniformly spaced recognition sites and BsmBI” thing (at least, the instance of it that I’ve found) looks rather sketchy and unconvincing to me, though I’m not really competent to evaluate it (are you?). It’s possible that what I’m looking at isn’t what you’re referring to; I’m talking about a post on Alex Washburne’s Substack where he draws attention to mention in the DEFUSE proposal of “reverse genetic systems” and “infectious clone technology” (though so far as I can see neither of these is actually mentioned in the proposal itself), claims (I do not know with what evidence) that using these methods would produce unusually regularly spaced instances of certain genome subsequences that are targeted by BsmBI or a similar enzyme, and claims that the SARS-CoV-2 shows such unusually regularly spaced instances.
But (1) unless there’s something I’m missing this “specifically links WIV to” those methods only in a very weak sense (e.g., the DEFUSE proposal doesn’t in fact say that they are going to use those methods, or that it would be done at the WIV), (2) Washburne doesn’t provide any support for his claim that researchers using this technique would in fact make the relevant segments unusually uniform in length, and (3) nor does he seem to give any details of the analysis that supposeedly shows that SARS-CoV-2 has such unusually uniform segments. He makes some claims about earlier drafts of the DEFUSE proposal supposedly obtained by FOIA requests, which if correct go some way to filling these gaps a bit, but if he actually shows those or gives evidence that they’re real then I haven’t seen it.
(Note: I find the style of Washburne’s writing very offputting; it pattern-matches to e.g. creationists crowing about their nonsensical statistical arguments. Lots of expressions of triumph, pointlessly flowery writing, that sort of thing. Of course that isn’t strong evidence that Washburne is wrong in the same sort of way as the creationists are, but I find it does strongly incline me to skepticism. It’s odd that, when arguing that something previously dismissed as a conspiracy theory is probably true, it doesn’t occur to him to try not writing like a conspiracy theorist.)
Daszak’s comments say that much of the work could be done at WIV which could include the FCS. The comment is genuine AFAIK but you’ll have to chase it up yourself.
The work on BsmBI is somewhat compelling because the people who suspected it published before DEFUSE was unearthed, and then DEFUSE was found to contain an order for BsmBI. So, they sort of predicted this. And there are reasons to make the virus out of relatively uniformly long segments—it’s convenient, the tools and techniques have maximum lengths they can handle, and you want to minimize the total amount of work. So you use roughly equal length segments. However this is a fairly complicated series of claims and I don’t see it as being the big win for lab-leak—the big win is DEFUSE itself.
But zoom out a bit: we can just notice that all of this stuff only collects around Wuhan, WIV and associated facilities like State Key Laboratory of Virology at Wuhan University.
There’s no equivalent of this in all the other cities in China and I am pretty sure that if you look through the top 20 cities by population you won’t find something like DEFUSE. I tried this a bit with chatGPT, Google Scholar, Google Search etc. There simply are not 50 other Daszaks out there doing GoF bat coronavirus research in every other city in China. Wuhan is THE PLACE where this happens.